Sex and stress steroid modulation of GABA mediated chloride ion flux in rat CNS
نویسندگان
چکیده
..................................................................................................... 6 ABBREVIATIONS....................................................................................... 7 LIST OF ORIGINAL PAPERS............................................................ 8 INTRODUCTION .......................................................................................... 9 Neuroactive steroids......................................................................................................................... 9 Synthesis................................................................................................................................................... 10 Allopregnanolone............................................................................................................................... 12 The clinical relevance of allopregnanolone.................................................................... 12 Neuroactive steroids and stress response ......................................................................... 13 The hormonal genomic pathway and the non-genomic pathway................... 15 Synaptic transmission................................................................................................................... 16 Neurotransmitters............................................................................................................................... 17 GABAA receptors.............................................................................................................................. 19 GABAA receptor function, agonists and antagonists............................................... 19 Subunits and distribution.............................................................................................................. 21 Kinetic properties of the GABAA receptor...................................................................... 21 AIMS.................................................................................................................... 24 MATERIAL AND METHODS ............................................................ 25 Animals........................................................................................................................................................ 25 Substances ................................................................................................................................................ 25 [S]TBPS binding assay............................................................................................................ 25 Chloride ion uptake assay........................................................................................................ 26 Patch-clamp recording................................................................................................................ 27 Statistical Analysis ........................................................................................................................... 28 RESULTS AND DISCUSSION ........................................................ 29
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